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| Registros recuperados: 23 | |
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| Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de. |
| The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082 |
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| Fayaz,Shima; Fard-Esfahani,Pezhman; Fard-Esfahani,Armaghan; Mostafavi,Ehsan; Meshkani,Reza; Mirmiranpour,Hossein; Khaghani,Shahnaz. |
| Homologous recombination (HR) is the major pathway for repairing double strand breaks (DSBs) in eukaryotes and XRCC2 is an essential component of the HR repair machinery. To evaluate the potential role of mutations in gene repair by HR in individuals susceptible to differentiated thyroid carcinoma (DTC) we used high resolution melting (HRM) analysis, a recently introduced method for detecting mutations, to examine the entire XRCC2 coding region in an Iranian population. HRM analysis was used to screen for mutations in three XRCC2 coding regions in 50 patients and 50 controls. There was no variation in the HRM curves obtained from the analysis of exons 1 and 2 in the case and control groups. In exon 3, an Arg188His polymorphism (rs3218536) was detected as a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA repair; Gene polymorphism; Mutation analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100004 |
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| Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney. |
| Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative... |
| Tipo: Text |
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf |
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| SCHNEIDER,MARCIA R.; SILVA,ANDRÉA L.G. DA; GOULART,CÁSSIA L.; SCHNEIDERS,PALOMA B.; WEBER,AUGUSTO F.; POSSUELO,LIA G.; VALIM,ANDREIA R.M.. |
| Abstract: This study evaluated 24 patients with lung cancer (CA) and 23 individuals with no smoking history or cancer in the family and without respiratory disease in childhood (CO). Peripheral blood lymphocytes was used to perform alkaline comet assay and to assess DNA damage as well as to evaluate methyl methane sulfonate (MMS) DNA repair after one hour and three hours at 37 ºC. The percentage of residual damage (RD) after three hours of MMS treatment, for each patient was assessed. The majority of patients were in the CA group, male patients, former smokers, with a history of smoking for 15 years and without associated comorbidities. Alkaline and residual damages were higher in the CA group when compared to controls (alkaline damage P = 0.015 and RD P =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; DNA repair; Lung cancer. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500806 |
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| ROCHA-MARTINS,MAURÍCIO; CAVALHEIRO,GABRIEL R.; MATOS-RODRIGUES,GABRIEL E.; MARTINS,RODRIGO A.P.. |
| ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Homologous recombination; DNA repair; Cre-LoxP; ZFN; TALEN; CRISPR/Cas9.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323 |
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| Chang,L.; Liu,Y.Y.; Zhu,B.; Li,Y.; Hua,H.; Wang,Y.H.; Zhang,J.; Jiang,Z.; Wang,Z.R.. |
| Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to 60Co-γ-rays. NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-γ-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Circadian; MPer2; Radiation; Cell death; Proliferation; DNA repair. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000003 |
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| Akcha, Farida; Burgeot, Thierry; Budzinski, H; Pfohla Leszkowicz, A; Narbonne, J. |
| With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg(-1) dry wt mussel d(-1)) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was... |
| Tipo: Text |
Palavras-chave: DNA repair; 8 oxodGuo; DNA adducts; Mussel; BaP. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-813.pdf |
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| Poplawski,Tomasz; Pastwa,Elzbieta; Blasiak,Janusz. |
| DNA double-strand breaks (DSBs) are a serious threat to the cell, for if not or miss-repaired, they can lead to chromosomal aberration, mutation and cancer. DSBs in human cells are repaired via non-homologous DNA end joining (NHEJ) and homologous recombination repair pathways. In the former process, the structure of DNA termini plays an important role, as does the genetic constitution of the cells, through being different in normal and pathological cells. In order to investigate the dependence of NHEJ on DSB structure in normal and cancer cells, we used linearized plasmids with various, complementary or non-complementary, single-stranded or blunt DNA termini, as well as whole-cell extract isolated from normal human lymphocytes, chronic myeloid leukemia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA repair; Non-homologous DNA end joining; DNA double-strand breaks; Complementary and non-complementary DNA ends. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200027 |
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| Cypriano,Ana Sheila; Alves,Gilda; Ornellas,Antonio Augusto; Scheinkman,José; Almeida,Renata; Scherrer,Luciano; Lage,Claudia. |
| Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prostate cancer; Single nucleotide polymorphism; XPD; DNA repair; Gene-environment interaction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500751 |
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| Cruz,Lavínia Almeida; Guecheva,Temenouga Nikolova; Bonato,Diego; Henriques,João Antônio Pêgas. |
| Eukaryotic cells have developed mechanisms to prevent genomic instability, such as DNA damage detection and repair, control of cell cycle progression and cell death induction. The bifunctional compound furocumarin 8-methoxy-psoralen (8-MOP) is widely used in the treatment of various inflammatory skin diseases. In this review, we summarize recent data about the role of chromatin remodeling in the repair of DNA damage induced by treatment with 8-methoxypsoralen plus UVA (8-MOP+UVA), focusing on repair proteins in budding yeast Saccharomyces cerevisiae, an established model system for studying DNA repair pathways. The interstrand crosslinks (ICL) formed by the 8-MOP+UVA treatment are detrimental lesions that can block transcription and replication, leading to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA repair; Psoralen; Chromatin remodeling; Histones; DNA interstrand cross-links. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600021 |
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| Registros recuperados: 23 | |
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